“Jason went for a scan this morning, all positive news, he won’t be fit for tomorrow,” England captain Eoin Morgan.
“We’ll monitor his progress in the week.
“To put one game on it I don’t think is sensible, let’s just see how it goes.”
Roy’s absence is a massive blow to England.
Brought into the side following the last World Cup, he averages 42.04 at the top of the order at a strike rate of 107.06.
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He has already hit a big century at the tournament against Bangladesh, as well as 54 against South Africa.
His replacement, James Vince, averages far lower with 26.50 while his strike-rate is far lower at 89.22. He has not formed part of England’s one-day revolution.
“It’s Jason Roy, of course he’s a big loss,” Morgan said.
“He’s an outstanding performer for us.
“I’m confident we have the batting covered. James Vince is an extremely talented, gifted player.”
“You can see that just watching him. We’ve every faith in him to go on and get a score at some stage.”
England must likely win two of their final three games against heavyweights Australia, India and New Zealand to guarantee their spot in the finals.
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However they could scrape in with one victory depending on other results.